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International journal of Immunopathology, allergology, infectology.

Expression of molecules of intercellular interaction on dendritic cells and T-lymphocytes in pulmonary tuberculosis patients

Urazova O.I., Esimova I.E., Khasanova R.R., Novitsky V.V., Churina E.G.

Siberian State Medical University, Russia, Tomsk

The work examines 107 patients with newly detected infiltrative and disseminated pulmonary TB before the start of etiotropic therapy. The objective of the research is to evaluate expression on dendritic cells (DC) and T-lymphocytes of surface molecules participating in the immune synapse formation. Using the method of flow cytometry, we carried out immunophenotyping of T-lymphocytes, isolated from blood and mature myeloid cells, transformed in vitro from blood monocytes, for the presence of the following markers: TCR/CD3, CD28, CTLA-4 (on T-lymphocytes) and HLA-DR, CD80, CD86 (on DC). It was demonstrated that apparent deficit of TCR/CD3+ lymphocytes, CD28+ -cells and DC, expressing co-stimulatory molecules of B7 family (CD80, CD86), was detected in TB patients. It is supposed, that, along with the deficit of molecules participating in intercellular cooperation and ensuring co-stimulation of the signal of their joint activation on DC and T-lymphocytes, disorders in anti-TB immune response may be mediated by immunosuppressor mechanisms, connected with an increased amount of CTLA-4+-cells with immunosuppressive activityand tolerogenic DC with the immunophenotype CD80+CD86-, detected in patients with pulmonary TB.

Keywords

Immune synapse, dendritic cells, T-lymphocytes, co-stimulatory molecules, suppression of immune response, pulmonary TB.

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Reference

Urazova O.I., Esimova I.E., Khasanova R.R., Novitsky V.V., Churina E.G. Immunopathology, allergology, infectology 2013; 4:17-21