Activation of the innate immunity factors in the patients after cardiac surgery with cardiopulmonary bypass
Vykhrystenka K.S., Smirnova A.U.
Vitebsk State Medical University, Vitebsk, Belarus
The aim of this research was to define changes of the innate immune factors (cytokines, adhesins, complement components) in the patients after cardiac surgery with cardiopulmonary bypass.
Material and methods. The study was performed by the protocol of open prospective study, which included patients with heart disease requiring surgery using cardiopulmonary bypass (CPB). We examined 15 patients. We studied blood cell count, blood biochemistry, indicators lymphocyte phenotype and activation markers (CD4 +, CD4 + CD25 +, CD8 +, CD8 + CD25 +, CD25 +, CD19 +), adhesion molecules on leukocytes (CR3, CR4, aÌ(CD11b), aÕ(CD11c), b2(CD18)), the levels of IL-1b, IL-2, IL-6, INF-g, C3 and C4 complement components, IgM, IgG, IgA. We studied these indicators before use CPB and one and 5-6 days after operation.
Results. The lymphocytes with markers CD4 + and CD8 + decreased practically twice in the first day after surgery and increased to previous values on the 5-6 days after surgery. The number of cells with a-chain for the IL-2 (CD25+) was changed by also. In the postoperative period CD19 + B lymphocytes was not significantly changed. In first day after surgery the IL-6 and IL-2 serum levels were increased and then their levels were returned to normal level to the 5-6 day. The IFN-g serum level was decreased to 5-6 day after surgery. The level of the compliment C3 component in serum increased in the first day after surgery and remained high. On first day after surgery the expression of adhesion molecules (aM (CD11b) and aX (CD11c)) were increased. Then their levels decreased, but were not normal to the 5-6-days.
Conclusions. The obtained results confirm activation of the innate immune factors of the patients after cardiac surgery with cardiopulmonary bypass.