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International journal of Immunopathology, allergology, infectology.

Đhronic urticaria and phenotyping of blood basophils by IgE-binding peptide

Yanchanka U.V.

Vitebsk State Medical University, Vitebsk, Belarus
JSC ôScientific-research enterprise Resanö, Vitebsk, Belarus

For phenotyping of leukocytes of patients with chronic urticaria and healthy volunteers were used monoclonal antibody and a synthetic analogue of the active center of a high affinity immunoglobulin E receptor (peptide p136-142FceRIa). To assess blood basophils we proposed new basophilic index of sensitization (BIS), which shows the percent of CD203˝+ from CD203˝+IgE+ basophils. From the synthetic peptide p136-142FceRIa, binding IgE, IgE identified differences between of basophils in patients with chronic urticaria and healthy volunteers. Patients with exacerbation of urticaria have the significant increase of basophilic index, which was 52.1 [44.4-62.5], while healthy people have 37.4 [31.3-40.0] (p <0.05). This change of BAI reflects the increased reaction potential of cells involved in type 1 allergic reactions. Patients suffering from urticaria have the increase of the number of basophils with IgE, bearing high affinity receptor immunoglobulin E, by almost 60%, in comparison with healthy people.
Basophilic index of sensitization (CD203˝+/CD203˝+IgE+) can be used as an objective immunologic marker of exacerbation of urticaria.

Keywords

Basophils, leukocytes, immunoglobulin E, synthetic peptide p136-142FceRIa, high affinity receptor for IgE, FceRIa, monoclonal antibodies, flow cytometry

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Reference

Yanchanka U.V. Immunopathology, allergology, infectology 2014; 2:19-23