Generation TNF-α, IL-1β in newly diagnosed patients with different clinical forms of pulmonary tuberculosis in the Astrakhan Region
Stepanova N.A., Galimzyanov Kh.M., Kantemirova B.I.
Astrakhan State Medical University Russian Ministry of Health
Objective: To study the spontaneous production of proinflammatory cytokines (TNF-α and IL-1β) in newly diagnosed patients with different clinical forms of pulmonary tuberculosis, Russian population groups living in the Astrakhan region.
Materials and Methods: on the basis of hospital ¹ 1 "Regional Clinical TB Dispensary" Astrakhan, performed a comprehensive clinical and immunological examination of 60 patients newly diagnosed with pulmonary tuberculosis. Men 44 (73.3%), 16 women (26.7%). Age – from 18 to 56 years. Residents of the city of Astrakhan was 29 (48.3%), rural area – 31 (51.7%). The level of proinflammatory cytokines in peripheral blood was measured before the start of a specific treatment, using a firm "VECTOR-BEST" Russia reagent kits.
Results. In a study comparing the two groups were formed: Ι – 31 patients with limited specific changes in lung tissue with a predominance of the productive component of inflammation; ΙΙ – 29 patients with bulky specific changes with the collapse of the lung tissue with a predominance of exudative inflammation component. In patients with disseminated tuberculosis patients Ι group serum TNF-α was detected in higher concentrations than patients ΙΙ group (8,16 ± 0,14 pg / ml vs. 4,01 ± 0,09 pg / ml; p <0.05 ). The same trend was observed at infiltrative tuberculosis patients, comparison groups, p <0.05. Development of IL-1β in serum, previously without a history of patients with tuberculosis compared groups is as follows: with disseminated - in group
Ι concentration 10,11 ± 1,07 ïã / ìë vs. 8,91 ± 1,07 pg / ml in group ΙΙ, p> 0.05; in infiltrative - in group Ι concentration 9,04 ± 0,94 pg / ml vs. 18,01 ± 1,04 pg / ml in group ΙΙ, p <0.05; when Tuberculomas only Ι group concentration was 6,81 ± 0,21 pg / ml; at the cavernous and fibro-cavernous pulmonary tuberculosis concentration was 9,15 ± 1,09 pg / ml.Conclusion. High concentrations of interleukin-1b potentiate the development of exudative-necrotic component specific inflammation and destruction of the lung tissue.