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International journal of Immunopathology, allergology, infectology.

Procedure of sterilisation and storage of histamine solutions with low concentrations

Prylutskyi O.S., Tkachenko K.Ye., Rogovaya Yu.D., Abylgazinova N.B.

Donetsk National Medical University named after Gorky, Donetsk, DPR

Background: study of the biological activity of histamine solutions after sterilization by autoclaving and membrane filtration as well as the assessment of the impact of single and repeated freezing and defrosting on the stability of prepared
solutions.
Material and methods. Histamine dihydrochloride solution at a concentration of 10 g/ml was sterilized using membrane filters or autoclaved at 121 C for 30 minutes, some of the samples were frozen in sterile insulin syringes, followed by 1- and 5-fold defrosting. Skin prick tests have been performed on one or both hands with the resulting solutions.
Results. Comparison of the results of skin prick tests performed with histamine solutions sterilized in various ways showed no statistically significant differences (p>0,05). No statistically significant differences between the results of trials performed with histamine solutions that hadn`t been frozen before, after 1- and 5-fold freezing and defrosting were found (p>0,05).
When comparing the results of skin prick tests performed no statistically significant differences were found (p>0.05).
Conclusion. It has been shown that autoclaving at 121 for 30 minutes followed by freezing and defrosting of histamine dihydrochloride solutions does not lead to a significant change in their activity.

Keywords

Histamine, reproducibility, sterilization, autoclaving,
membrane filtration.

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Reference

Prylutskyi O.S., Tkachenko K.Ye., Rogovaya Yu.D., Abylgazinova N.B. Immunopathology, allergology, infectology 2017; 4:44-49

DOI

10.14427/jipai.2017.4.53