Features of the cytokine profile of blood serum and cerebrospinal fluid in patients with traumatic brain injury
Dyukarev V.V., Yudina S.M., Korolev A.G.
Kursk State Medical University, Kursk, Russia
The aim of the work is to study the cytokine profile of blood serum and cerebrospinal fluid in patients with severe traumatic brain injury (TBI). Materials and methods. The study included 20 patients with TBI on the Glasgow Coma Scale ≤8. The collection of cerebrospinal fluid (CSF) and blood serum was performed on days 1 and 7 after trauma. The content of cytokines (IL-1β, TNF-α, IL-6, IL-8, IFN-γ and IL-10) in serum and CSF was determined by solid-phase IFA with a set of reagents of firm "Vector-Best" (Novosibirsk). Results. Severe TBI is accompanied by hypersecretion of pro- and anti-inflammatory cytokines whose production in the dynamics of the development of the inflammatory process that occurs when traumatic brain tissue lesions is characterized by a significant increase in their concentration in the first day and a decrease in dynamics on the 7th day after TBI except IL-6 and IL-8, the content of which increased in both biological fluids by 1.5-2 times, respectively. In this case, the level of cytokines in the CSF significantly exceeded their serum values by an average of 1.5 times. A direct correlation between the level of IL-6, IL-8, cytosis and protein content in the CSF was revealed, indicating their important role in the pathogenesis of TBI, which justifies the feasibility of their investigation for predicting the course and evaluating the effectiveness of treatment.