Amino acid homeostasis in the implementation of adaptive responses in animals with
different resistance to tuberculosis infection
Sabadash E.V., Skornyakov S.N., Novikov B.I., Pavlov V.A.
Ural Research Institute for Phthisiopulmonology, a branch of “National Medical Research Center of Phthisiopulmonology and Infectious Diseases” Ministry of Healthcare of Russian Federation
Objective: in an experiment to identify typical reactions of amino acid homeostasis under the damaging effects of various origins.
Methods. The experiments were carried out on laboratory animals - rats and guinea pigs. The choice of two models of experimental tuberculosis was due to different resistance to this infection. Three options for exposure to them were studied: 1) infection with Mycobacterium tuberculosis (MBT); 2) infection of the office in combination with the subsequent end tracheal administration of polycyclic aromatic hydrocarbons (PAHs) adsorbed on soot in the form of an oil solution of coal tar; 3) infection of the office in combination with acute blood loss. Animals were divided into 8 groups of 20 animals each. Control groups (No. 1 and No. 2) - intact guinea pigs and rats. All other animals of the experimental groups (6) were infected with the museum strain MBT H3RV: guinea pigs at a dose of 0.001 g to the inguinal fold, rats at a dose of 0.1 g to the tail vein. In all infected MBT animals, after 2 weeks, the parameters characteristic of experimental tuberculosis were studied. Experimental groups No. 3 (guinea pigs) and No. 4 (rats) were not exposed to any other exposure to MBT, Groups No. 5 (guinea pigs) and No. 6 (rats) were subjected to the combined effects of MBT infection and end tracheal administration of mutagenic and carcinogenic PAHs adsorbed on soot in the form of an oil solution of coal tar. In groups No. 7 (guinea pigs) and No. 8 (rats), the animals were infected with MBT in combination with acute blood loss: bloodletting was performed from the femoral artery - 2.5% of the blood weight. In animals of each group, amino acids and glutathione were tested.
Results. It has been established that in the tissues (liver, etc.) of rats, an increased level of antioxidant metabolites (ascorbic acid, taurine, glutathione) probably determines their resistance to pathological effects such as infection of the office, toxic effects, but not states associated with deep posthaemorrhagic hypoxia.