CD3+CD4+CD25+ activated T cells and CD3+CD4+CD25+highCD127+low T-regulatory
lymphocytes in patients after kidney transplantation with various types
of post-transplantation period
Republican Research Center for Radiation Medicine and Human Ecology, Gomel, Belarus
Objective. To study the dynamics of CD3+CD4+CD25+ (activated T-helper cell) and CD3+CD4+CD25+highCD127+low (regulatory T cell) lymphocytes in kidney transplant recipients (KTR) with diff erent course of post-transplantation period. Methods. Out of 165 recipients of a renal allograft , 4 groups were formed. Th e fi rst group (KTR1) included patients with satisfactory primary and delayed renal transplant functions, the second group (KTR2) had satisfactory primary function and delayed graft dysfunction, the third group (KTR3)included primary dysfunction and delayed satisfactory function, the fourth group (KTR4) included patients with primary and delayed transplant dysfunction. Th e number of CD3+CD4+CD25+ è CD3+CD4+CD25+highCD127+low in the blood was determined at the 0th, 1st, 3rd, 10th, 30th, 90th, 180th, 360th day.
Results and Discussion. Th ere were no signifi cant differences in the number of CD3+CD4+CD25+ lymphocytes between patients prior to kidney transplantation and the comparison group. In turn, the number of CD3+CD4+CD25+highCD127+low T-lymphocytes in the group with the most favorable post-transplant period prior to surgery was significantly lower than in the comparison group with a subsequent decrease in the response to anti-CD25 antibody therapy. Aft er a year of observation, this indicator recovered to the level of the comparison group, which was not observed in other groups.
In KTR3 group, the primary function was not satisfactory, but aft er 360 days the creatinine level was below 150 μmol/L, and the number of the regulatory CD3+CD4+CD25+highCD127+low subpopulation was lower than the comparison group, but not lower than the absolute number in the group with stable satisfactory function of KTR1 transplant.
Conclusion. Th e recovery of the number of CD3+CD4+CD25+ è CD3+CD4+CD25+highCD127+low lymphocytes on the 90th day with a stable level throughout the year is typical for patients with satisfactory primary and delayed renal transplant function. In the group with primary dysfunction and delayed satisfactory renal transplant function, the number of the regulatory subpopulation CD3+CD4+CD25+highCD127+low had a positive tendency and there were no significant differences in the absolute number with the group of patients with satisfactory primary and delayed function on the 360th day.