Molecular profile of sensitization to dog allergens: diagnostics using the “AllergochipRF” platform

Kozlov E.M., Trifonova D.R., Ryabova K.A., Dubovets A.A., Vintsevskaya A.M., Lukashevichus A.D., Baskakov A.A., Fomina D.S., Evsegneeva I.V., Karaulov A.V.

Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation, Moscow, Russia
Laboratory of Immunology and Infectology, LIFT Science Center (Life Improvement by Future Technologies), Skolkovo Innovation Center, Moscow, Russia
Medical University of Vienna, Vienna, Austria
City Clinical Hospital №52, Moscow, Russia

The aim of this study was to assess sensitization to molecular dog allergens Can f 1 – Can f 6 in patients with clinical manifestations of bronchial asthma, allergic rhinitis, conjunctivitis, and/or atopic dermatitis using the diagnostic platform “AllergochipRF.” Recombinant allergens Can f 1, Can f 2, Can f 4, Can f 5, and Can f 6 were expressed in a heterologous Escherichia coli-based system and purified by metal-chelate affinity chromatography. The secondary structure of the obtained proteins was characterized using circular dichroism spectroscopy. The study included 100 patients with allergy symptoms, of whom 64% were found to be sensitized to at least one of the Can f 1 – Can f 6 components. The results showed that the major allergens in dog allergy are the molecular allergens Can f 1, Can f 5, and Can f 6. These findings confirm the clinical relevance of molecular diagnostics for dog allergen sensitization and highlight the key role of these components in allergic response development.