Standardization of cigarette smoke extract for provocative test

Ishchanka A.U., Khaitov M.R., Hardziyevich T.H., Shilovskiy I.P., Goncharova T.N., Yatsko M.V.

Vitebsk State Medical University, Vitebsk, Belarus

Aim. Determination of nicotine concentration by gas chromatography with a flame ionization detector for standardization of cigarette smoke extract (CSE).
Materials and methods. Nicotine analysis in CSE samples was performed on a DANI Master GC gas chromatograph equipped with a flame ionization detector. CSE generation and collection were performed on a patented device (patent BY 11866 dated 30.12.2018). Cigarettes were used as a source for CSE, with a nicotine content of 0,7 mg/cigarette, tar 8 mg/cigarette. The analytical standard used was (-) - nicotine, ≥99% (GC), the internal standard was hexadecane, 99,15% (g/g) ± 0,3% (g/g), the solvent was hexane P, and nitrogen for chromatography P was used as the mobile phase. Blank solutions, calibration solutions and solutions of the CSE test sample were introduced into the gas chromatograph at 1 μl, obtaining at least one chromatogram for each solution. At the end of the analysis, the data were recorded and the quantitative content of nicotine in the test sample CSE (mg/ml) was calculated.
Results. Using gas chromatography with a flame ionization detector, the concentration of nicotine in CSE was determined to be from 0,029 to 0,031 mg/ml.
Conclusion. A method of gas chromatography with a flame ionization detector for quantitative determination of nicotine content in a CSE has been developed. The method is suitable and applicable for the analysis of CSE, making it possible to standardize the CSE for nicotine for subsequent use for research and diagnostic purposes.